边居
单元格排序
癌症干细胞
流出
流式细胞术
干细胞
生物
细胞生物学
人口
分子生物学
化学
医学
生物化学
环境卫生
作者
Masayuki Shimoda,Masahide Ota,Yasunori Okada
出处
期刊:Methods in molecular biology
日期:2017-10-07
卷期号:: 49-59
被引量:34
标识
DOI:10.1007/978-1-4939-7401-6_5
摘要
The Hoechst side population (SP) method is a flow cytometry technique used to obtain stem cells based on the dye efflux properties of the ATP-binding cassette (ABC) transporters. The SP cells are characterized by their capability to efflux the fluorescent DNA-binding dye Hoechst 33342 through their ABC transporters and are enriched in stem cells, which are endowed with a self-renewal capacity and multilineage differentiation potential and express the stemness genes including ABC multidrug transporters. The protocols outlined in this book chapter describe the isolation method of the SP cells from human lung carcinoma cell lines by using Hoechst 33342. In addition, we refer to the propagation method of SP cells by successive rounds of fluorescence-activated cell sorting analysis for SP cells. These approaches will be helpful for the establishment of novel in vitro and in vivo models using cancer stem cells, which may play a key role during carcinogenesis and/or tumor progression.
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