Multifunctional nanocomplex for surface-enhanced Raman scattering imaging and near-infrared photodynamic antimicrobial therapy of vancomycin-resistant bacteria

Since vancomycin (Van)-resistant enterococci (VRE) strains first emerged as a serious threat to public health, extensive studies focused on optical imaging and antimicrobial therapy have been performed for monitoring and microbiological control. In this study, we developed silicon 2,3-naphthalocyanine dihydroxide (Nc) and Van functionalized silica-encapsulated, silver-coated gold nanoparticles (Au@AgNP@SiO2@Nc-Van) as a novel theranostic system for surface-enhanced Raman scattering (SERS) detection and antimicrobial photodynamic therapy (aPDT) of VRE strains. The silver-coated gold nanoparticle, as the SERS-active core, exhibited excellent Raman enhancement efficacy. Results of in vitro bacterial SERS imaging revealed Van-enhanced specific binding affinity toward VRE. Meanwhile, Si(IV) naphthalocyanine, serving as a near-infrared (NIR) photosensitizer, was axially linked to the nanoparticle surface, yielding nanostructured hybrid materials that could photoinactivate VRE. Almost 4-5 logs of bacterial reduction were obtained upon in vitro photodynamic therapy of VRE treated with a nanomolar concentration of the nanocomplex. Mouse infection assays were applied for an in vivo evaluation of VRE lethality. Upon near-infrared light irradiation, this hybrid nanomaterial caused obvious infection regression and even complete eradication compared to the findings in the non-treated groups. Therefore, this novel nanosystem integrating SERS imaging and noninvasive aPDT has huge potential for applications in theranostics with regard to VRE management.