A dual-response fluorescent probe for discriminative sensing of hydrazine and bisulfite as well as intracellular imaging with different emission

Bisulfite and hydrazine are harmful to the environment safety and human health. Therefore, it is of great value to develop a smart fluorescent probe with high selectivity for detection of bisulfite and hydrazine. In our report, a dual-response fluorescent probe EDBI with high selectivity, rapid response, and low detection limit for discriminative determination HSO3- and N2H4 was exploited. The probe EDBI is capable of distinctive sensing HSO3- and N2H4 based on nucleophilic addition reactions by taking advantage of ratiometric fluorescence and fluorescence "on-off" mode, respectively. The dual-responses behaviors of probe EDBI toward HSO3- and N2H4 were attribute to different reaction sites, which it has been confirmed by HRMS. More importantly, cytotoxicity experiment authenticated that probe possesses low toxicity and good penetration. The probe EDBI with excellent performance, it was successfully employed to distinguishable sense HSO3- and N2H4 in living cells by diverse channel patterns. Therefore, this simple dual-response fluorescence probe is expected to be used for real-time monitoring bisulfite and hydrazine in biological samples.