产甲烷
16S核糖体RNA
厌氧消化
微生物种群生物学
核糖体RNA
生物
丰度(生态学)
甲烷
古细菌
微生物学
细菌
生态学
生物化学
基因
遗传学
作者
Qiang Lin,Jo De Vrieze,Jiabao Li,Xiangzhen Li
标识
DOI:10.1016/j.biortech.2016.02.132
摘要
Temperature is a major factor determining the performance of the anaerobic digestion process. The microbial abundance, activity and interactional networks were investigated under a temperature gradient from 25°C to 55°C through amplicon sequencing, using 16S ribosomal RNA and 16S rRNA gene-based approaches. Comparative analysis of past accumulative elements presented by 16S rRNA gene-based analysis, and the in-situ conditions presented by 16S rRNA-based analysis, provided new insights concerning the identification of microbial functional roles and interactions. The daily methane production and total biogas production increased with temperature up to 50°C, but decreased at 55°C. Increased methanogenesis and hydrolysis at 50°C were main factors causing higher methane production which was also closely related with more well-defined methanogenic and/or related modules with comprehensive interactions and increased functional orderliness referred to more microorganisms participating in interactions. This research demonstrated the importance of evaluating functional roles and interactions of microbial community.
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