A novel therapeutic combination of dapagliflozin, Lactobacillus and crocin attenuates diabetic cardiomyopathy in rats: Role of oxidative stress, gut microbiota, and PPARγ activation

糖尿病性心肌病 达帕格列嗪 医学 糖尿病 氧化应激 药理学 肠道菌群 化学 番红花苷 内科学 心肌病 内分泌学 2型糖尿病 生物化学 心力衰竭
作者
Eman M. Khalaf,Hanan M. Hassan,Ahmed M. El-Baz,Ahmed Shata,Ahmed E. Khodir,Mahmoud E. Yousef,Rehab Mohamed Elgharabawy,Nehal A. Nouh,Safaa Saleh,Mashael Mohammed Bin-Meferij,Attalla F. El‐kott,Mohamed Mohamed Adel El‐Sokkary,Hanan Eissa
出处
期刊:European Journal of Pharmacology [Elsevier]
卷期号:931: 175172-175172 被引量:13
标识
DOI:10.1016/j.ejphar.2022.175172
摘要

Diabetic cardiomyopathy is diagnosed by the development of abnormality in the structure and performance of myocardium in diabetic mellitus (DM) patients. Recent studies reported the association between altered gut microbiota and metabolic disorders like diabetes and cardiovascular diseases. Here, we aimed to investigate the gut-heart axis in an experimental animal model where we developed a novel therapeutic combination of dapagliflozin, crocin prebiotic and Lactobacilli probiotic to correct induced diabetic cardiomyopathy.Diabetes mellitus was induced by Intraperitoneal (i.p) streptozotocin in male rats. The experimental design includes the administration of the tested drugs (Crocin, Dapagliflozin) solely and with Lactobacillus, or in combination therapy with and without Lactobacillus to the diabetic rats for six weeks. Clinical and microscopic evaluation scoring for cardiac tissues were determined. Biochemical markers including blood glucose level, adiponectin, resistin, cardiac injury markers, lipid profile, antioxidant enzymes, pro and anti-inflammatory markers were assessed. In addition, quantitative relative expression of PPARγ and TXINP genes and capsase-3 levels were measured. The change in the microbiota abundance was investigated using real-time PCR.This study demonstrated the synergistic effect of the triple combination; dapagliflozin, crocin prebiotic, and Lactobacillus fermentum and Lactobacillus delbrueckii probiotic in treating diabetic cardiomyopathy in rats. The triple combination significantly reduced the oxidative, inflammatory, apoptotic activities induced by streptozotocin STZ and helped in restoring the symbiotic gut microbiota.It is worthy to perform this study in clinical trials as a primary step to include crocin and Lactobacilli in the therapeutic protocols of diabetic cardiomyopathy.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
wjm发布了新的文献求助10
刚刚
刚刚
雪寒完成签到,获得积分10
刚刚
Owen应助swordlee采纳,获得10
刚刚
小平头啤酒肚完成签到,获得积分10
1秒前
咿咿呀呀发布了新的文献求助10
1秒前
Lucas应助Nightfall采纳,获得10
1秒前
1秒前
2秒前
高高的汝燕完成签到,获得积分10
2秒前
悦耳凡柔发布了新的文献求助20
3秒前
max发布了新的文献求助10
3秒前
Anxietymaker发布了新的文献求助10
4秒前
完美世界应助王京华采纳,获得10
4秒前
西西完成签到,获得积分10
6秒前
彩虹天堂发布了新的文献求助10
7秒前
8秒前
8秒前
玛卡巴卡发布了新的文献求助10
8秒前
9秒前
咿咿呀呀完成签到,获得积分10
9秒前
Anxietymaker完成签到,获得积分10
10秒前
biov完成签到,获得积分10
10秒前
LYY完成签到,获得积分10
11秒前
11秒前
狂野怜蕾发布了新的文献求助10
11秒前
12秒前
qq.com发布了新的文献求助10
13秒前
13秒前
123发布了新的文献求助10
14秒前
谨慎含双发布了新的文献求助10
14秒前
李健的小迷弟应助gyhk采纳,获得10
16秒前
16秒前
16秒前
MOON完成签到,获得积分10
16秒前
郁离子完成签到 ,获得积分10
17秒前
成就雨筠应助斯文采纳,获得10
17秒前
着急的初丹完成签到,获得积分10
17秒前
Yancy给罗小姐的求助进行了留言
18秒前
19秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Mechanistic Modeling of Gas-Liquid Two-Phase Flow in Pipes 2500
Kelsen’s Legacy: Legal Normativity, International Law and Democracy 1000
Handbook on Inequality and Social Capital 800
Conference Record, IAS Annual Meeting 1977 610
Interest Rate Modeling. Volume 3: Products and Risk Management 600
Interest Rate Modeling. Volume 2: Term Structure Models 600
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3546508
求助须知:如何正确求助?哪些是违规求助? 3123622
关于积分的说明 9356111
捐赠科研通 2822292
什么是DOI,文献DOI怎么找? 1551314
邀请新用户注册赠送积分活动 723303
科研通“疑难数据库(出版商)”最低求助积分说明 713699