生物
阿尔戈瑙特
反式siRNA
掷骰子
小干扰RNA
RNA诱导的转录沉默
基因沉默
细胞生物学
RNA沉默
RNA诱导沉默复合物
核糖核酸
抄写(语言学)
分子生物学
RNA干扰
遗传学
基因
哲学
语言学
作者
Jing Li,Brandon H. Le,Xufeng Wang,Ye Xu,Suikang Wang,Hao Li,Lei Gao,Beixin Mo,Lin Liu,Xuemei Chen
标识
DOI:10.1093/plcell/koae293
摘要
Abstract RNA silencing negatively regulates gene expression at the transcriptional and post-transcriptional levels through DNA methylation, histone modification, mRNA cleavage, and translational inhibition. Small interfering RNAs (siRNAs) of 21 to 24 nucleotides are processed from double-stranded RNAs by Dicer-like enzymes and play essential roles in RNA silencing in plants. Here, we demonstrated that ALTERED MERISTEM PROGRAM1 (AMP1) and its putative paralog LIKE AMP1 (LAMP1) impair RNA silencing by repressing the biogenesis of a subset of inverted repeat (IR)–derived siRNAs in Arabidopsis (Arabidopsis thaliana). AMP1 and LAMP1 inhibit Pol II-dependent IR gene transcription by suppressing ARGONAUTE 1 (AGO1) protein levels. Genetic analysis indicates that AMP1 acts upstream of RNA polymerase IV subunit 1 (NRPD1), RNA-dependent RNA polymerase 2 (RDR2), and Dicer-Like 4 (DCL4), which are required for IR-induced RNA silencing. We also show that AMP1 and LAMP1 inhibit siRNA-mediated silencing in a different mechanism from AGO4 and DCL3. Together, these results reveal two previously unknown players in siRNA biogenesis from IRs – AGO1, which promotes IR transcription, and AMP1, which inhibits IR transcription indirectly through the repression of AGO1 expression.
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