单甘醇
染色质免疫沉淀
表观遗传学
组蛋白
生物
生物化学
木质素
发起人
乙酰化
染色质
细胞生物学
生物合成
化学
基因表达
基因
植物
作者
Hongyan Ma,Li‐Wei Su,Wen Zhang,Yi Sun,Danning Li,Shuang Li,Ying‐Chung Jimmy Lin,Chenguang Zhou,Wei Li
摘要
Summary Lignin, a major wood component, is the key limiting factor for wood conversion efficiency. Its biosynthesis is controlled by transcriptional regulatory networks involving transcription factor (TF)–DNA interactions. However, the epigenetic mechanisms underlying these interactions in lignin biosynthesis remain largely unknown. Here, using yeast one‐hybrid, chromatin immunoprecipitation, and electrophoretic mobility shift assays, we identified that PtrbZIP44‐A1, a key wood‐forming TF, directly interacts with the promoters of PtrCCoAOMT2 and PtrCCR2 , genes involved in the monolignol biosynthetic pathway. We used yeast two‐hybrid, bimolecular fluorescence complementation, biochemical analyses, transient and CRISPR‐mediated transgenesis in Populus trichocarpa to demonstrate that PtrHDA15, a histone deacetylase, acts as an epigenetic inhibitor and is recruited by PtrbZIP44‐A1 for chromatin histone modifications to repress PtrCCoAOMT2 and PtrCCR2 , leading to reduced lignin deposition. In transgenic lines overexpressing PtrbZIP44‐A1 or PtrHDA15 , histone acetylation at the promoters of PtrCCoAOMT2 and PtrCCR2 decreased, reducing their expression and lignin content. Conversely, in loss‐of‐function ptrbzip44‐a1 and ptrhda15 mutants, histone acetylation levels at PtrCCoAOMT2 and PtrCCR2 promoters increased, enhancing target gene expression and lignin content. Our study uncovered an epigenetic mechanism that suppresses lignin biosynthesis. This finding may help fill a knowledge gap between epigenetic regulation and lignin biosynthesis during wood formation in Populus .
科研通智能强力驱动
Strongly Powered by AbleSci AI