血小板
P-选择素
内皮
活体显微镜检查
粘附
血小板粘附
血小板活化
选择素
化学
股动脉
炎症
细胞生物学
内皮干细胞
免疫学
医学
生物
内科学
体外
生物化学
微循环
血小板聚集
有机化学
作者
Öscar Braun,Jan E. Slotta,Michael D. Menger,David Erlinge,Henrik Thorlacius
标识
DOI:10.1016/j.ejphar.2008.06.102
摘要
Platelets constitute a key role in vascular injuries, however, the detailed mechanisms behind platelet–endothelial cell and platelet–leukocyte interactions in the femoral artery are not yet fully elucidated. We used intravital fluorescence microscopy of the femoral artery in C57BL/6 mice to study primary and secondary capture of platelets onto endothelial cells as well as onto adherent platelets and leukocytes in vivo. By use of monoclonal antibodies, the role of P-selectin and P-selectin glycoprotein ligand 1 (PSGL-1) in these adhesive interactions in mice exposed to endotoxin was determined. Intravenous injection of endotoxin significantly increased gene expression of P-selectin as well as platelet tethering, rolling and adhesion in the femoral artery. Pretreatment with the anti-PSGL-1 antibody decreased platelet tethering by 85%, platelet rolling by 88% and platelet adhesion by 96%. Immunoneutralization of P-selectin reduced platelet tethering by 91%, platelet rolling by 98%, and platelet adhesion by 97%. In addition, inhibition of P-selectin and PSGL-1 completely abolished secondary capture of platelets onto adherent platelets and leukocytes. Our data show that P-selectin and PSGL-1 mediate early interactions between platelets and other cells, including endothelial cells and leukocytes, in inflamed arteries. These novel results suggest that interference with P-selectin and PSGL-1 may be a useful target in strategies aiming to protect the vascular wall during arterial inflammation.
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