葡萄糖氧化酶
表面改性
Nafion公司
电化学
化学
电极
过硫酸铵
电催化剂
羧化
核化学
无机化学
化学工程
有机化学
催化作用
聚合物
聚合
工程类
物理化学
作者
Chuhan Lv,Shuangfei Li,Liangxu Liu,Xingyu Zhu,Xiao Yang
出处
期刊:Sensors
[MDPI AG]
日期:2020-06-13
卷期号:20 (12): 3365-3365
被引量:8
摘要
This research revealed the effect of carboxyl-functionalization on the mesoporous carbon (MC)-fixed glucose oxidase (GOx) for promoting the properties of bioelectrodes. It showed that the oxidation time, temperature and concentration, can significantly affect MC carboxylation. The condition of 2 M ammonium persulfate, 50 °C and 24 h was applied in the study for the successful addition of carboxyl groups to MC, analyzed by FTIR. The nitrogen adsorption isotherms, and X-ray diffraction analysis showed that the carboxylation process slightly changed the physical properties of MC and that the specific surface area and pore size were all well-maintained in MC-COOH. Electrochemical characteristics analysis showed that Nafion/GOx/MC-COOH presented better electrocatalytic activity with greater peak current intensity (1.13-fold of oxidation peak current and 4.98-fold of reduction peak current) compared to Nafion/GOx/MC. Anodic charge-transfer coefficients (α) of GOx/MC-COOH increased to 0.77, implying the favored anodic reaction. Furthermore, the GOx immobilization and enzyme activity in MC-COOH increased 140.72% and 252.74%, leading to the enhanced electroactive GOx surface coverage of Nafion/GOx/MC-COOH electrode (22.92% higher, 1.29 × 10−8 mol cm−2) than the control electrode. Results showed that carboxyl functionalization could increase the amount and activity of immobilized GOx, thereby improving the electrode properties.
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