再狭窄
间充质干细胞
支架
材料科学
内膜增生
生物医学工程
聚赖氨酸
医学
外科
平滑肌
病理
生物
内科学
生物化学
作者
Xue Wu,Guixue Wang,Chaojun Tang,Dechuan Zhang,Zhenggong Li,Dingyuan Du,Zhengcai Zhang
摘要
Abstract This study is designed to make a novel cell seeding stent and to evaluate reendothelialization and anti‐restenosis after the stent implantation. In comparison with cell seeding stents utilized in previous studies, Mesenchymal stem cells (MSCs) have advantages on promoting of issue repair. Thus it was employed to improve the reendothelialization effects of endovascular stent in present work. MSCs were isolated by density gradient centrifugation and determined as CD29 + CD44 + CD34 − cells by immunofluorescence and immunocytochemistry; gluten and polylysine coated stents were prepared by ultrasonic atomization spray, and MSCs seeded stents were made through rotation culture according to the optimized conditions that were determined in previous studies. The results from animal experiments, in which male New Zealand white rabbits were used, show that the reendothelialization of MSCs coated stents can be completed within one month; in comparison with 316L stainless steel stents (316L SS stents) and gluten and polylysine coated stents, the intimal hyperplasia and in‐stent restenosis are significantly inhibited by MSCs coated stents. Endovascular stent seeded with MSCs promotes reendothelialization and inhibits the intimal hyperplasia and in‐stent restenosis compared with the 316L SS stents and the gluten and polylysine coated stents. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: , 2011.
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