微生物学
双歧杆菌
短双歧杆菌
生物
长双歧杆菌
益生菌
细菌
毒力
肠道菌群
耶尔森菌病
粪便
双歧杆菌
腹泻
乳酸菌
抗生素
病菌
致病菌
肠杆菌科
大肠杆菌
生物化学
遗传学
基因
作者
Cristina Delcaru,Ionela Alexandru,Paulina Podgoreanu,Violeta Corina Cristea,Coralia Bleoţu,Mariana Carmen Chifiriuc,Eugenia Bezirtzoglou,Veronica Lazăr
出处
期刊:Anaerobe
[Elsevier]
日期:2016-06-01
卷期号:39: 39-44
被引量:28
标识
DOI:10.1016/j.anaerobe.2016.02.010
摘要
The gastrointestinal microbiota contributes to the consolidation of the anti-infectious barrier against enteric pathogens. The purpose of this study was to investigate the influence of Bifidobacterium sp. strains, recently isolated from infant gastrointestinal microbiota on the in vitro growth and virulence features expression of enteropathogenic bacterial strains. The antibacterial activity of twelve Bifidobacterium sp. strains isolated from human feces was examined in vitro against a wide range of Gram negative pathogenic strains isolated from 30 infant patients (3 days to 5 years old) with diarrhea. Both potential probiotic strains (Bifidobacterium longum, Bifidobacterium pseudocatenulatum, Bifidobacterium catenulatum, Bifidobacterium breve, Bifidobacterium ruminantium) and enteropathogenic strains (EPEC, EIEC, Klebsiella pneumoniae, Salmonella sp., Yersinia enterocolitica, Pseudomonas aeruginosa) were identified by MALDI-TOF and confirmed serologically when needed. The bactericidal activity, growth curve, adherence to the cellular HEp-2 substratum and production of soluble virulence factors have been assessed in the presence of different Bifidobacterium sp. cultures and fractions (whole culture and free-cell supernatants). Among the twelve Bifidobacterium sp. strains, the largest spectrum of antimicrobial activity against 9 of the 18 enteropathogenic strains was revealed for a B. breve strain recently isolated from infant intestinal feces. The whole culture and free-cell supernatant of B. breve culture decreased the multiplication rate, shortened the log phase and the total duration of the growth curve, with an earlier entrance in the decline phase and inhibited the adherence capacity to a cellular substratum and the swimming/swarming motility too. These results indicate the significant probiotic potential of the B. breve strain.
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