蛋白质组学
网格蛋白
细胞生物学
生物
亚细胞定位
高尔基体
定量蛋白质组学
信号转导衔接蛋白
突触小泡
蛋白质亚细胞定位预测
网格蛋白接合器蛋白
细胞分离
细胞器
蛋白质组
细胞室
膜蛋白
细胞培养中氨基酸的稳定同位素标记
小泡
生物化学
细胞质
信号转导
内质网
膜
基因
细胞
作者
Brigitte Ritter,F Blondeau,A. Yu. Denisov,Kalle Gehring,Peter S. McPherson
出处
期刊:Biochemical Society Transactions
[Portland Press]
日期:2004-10-26
卷期号:32 (5): 769-773
被引量:25
摘要
Subcellular proteomics is a powerful new approach that combines subcellular fractionation and MS (mass spectrometry) to identify the protein complement of cellular compartments. The approach has been applied to isolated organelles and major suborganellar structures and each study has identified known proteins not previously understood to associate with the compartment and novel proteins that had been described only as predicted open-reading frames from genome sequencing data. We have utilized subcellular proteomics to analyse the protein components of CCVs (clathrin-coated vesicles) isolated from adult brain. Accounting for identified fragmented peptides allows for a quantitative assessment of protein complexes associated with CCVs, and the identification of many of the known components of post-fusion synaptic vesicles demonstrates that a main function for brain CCVs is to recycle synaptic vesicles. In addition, we have identified a number of novel proteins that participate in CCV formation and function at the trans-Golgi network and the plasma membrane. Characterization of two of these proteins, NECAP1 and NECAP2, has led to the identification of a new consensus motif that mediates protein interactions with the clathrin adaptor protein 2. These studies highlight the ability of proteomics to reveal new insights into the mechanisms and functional roles of subcellular compartments.
科研通智能强力驱动
Strongly Powered by AbleSci AI