Cry1Ac公司
苏云金杆菌
生物杀虫剂
中肠
生物
苏力菌棉花
食品科学
化学
转基因作物
植物
杀虫剂
农学
生物化学
细菌
转基因
遗传学
幼虫
基因
作者
Xiaohong Pan,Fang Cao,Xueping Guo,Yilin Wang,Zhanglin Lin Cui,Tianpei Huang,Youming Hou,Xiong Guan
标识
DOI:10.1021/acs.jafc.4c00833
摘要
Mg(OH)2 was used as the nanocarrier of the Bacillus thuringiensis (Bt) Cry1Ac protein, and the synthesized Cry1Ac–Mg(OH)2 composites were regular and uniform nanosheets. Nano-Mg(OH)2 could effectively improve the insecticidal effect of the Cry1Ac protein toward Ectropis obliqua. It could enhance the damage degree of the Cry1Ac protein to intestinal epithelial cells and microvilli, induce and enrich the production of reactive oxygen species (ROS) in the midgut, and enhance the degradation of the Cry1Ac protein into active fragments. Furthermore, an anti-rinsing assay showed that the Cry1Ac–Mg(OH)2 composites were bound to the notch structure of the tea leaf surface. The retention of the Cry1Ac protein increased by 11.45%, and sprayed nano-Mg(OH)2 was rapidly absorbed by different tissues of tea plants. Moreover, nano-Mg(OH)2 and composites did not significantly affect non-target organisms. These results show that nano-Mg(OH)2 can serve as a safe and effective biopesticide carrier, which provides a new approach for stable and efficient Bt preparation.
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