形态学(生物学)
牵引(地质)
显微镜
生物物理学
材料科学
解剖
生物
物理
动物
光学
古生物学
作者
Yuanyuan Tao,Ajinkya Ghagre,Clayton Molter,Anna Clouvel,Jalal Al Rahbani,Claire M. Brown,Derek Nowrouzezahrai,Allen J. Ehrlicher
标识
DOI:10.1016/j.bpj.2024.07.020
摘要
Traction Force Microscopy (TFM) has emerged as a widely used standard methodology to measure cell-generated traction forces and determine their role in regulating cell behavior. While TFM platforms have enabled many discoveries, their implementation remains limited due to complex experimental procedures, specialized substrates, and the ill-posed inverse problem where low magnitude high-frequency noise in the displacement field severely contaminates the resulting traction measurements. Here, we introduce Deep Morphology Traction Microscopy (DeepMorphoTM), a Deep Learning alternative to conventional TFM approaches. DeepMorphoTM first infers cell-induced substrate displacement solely from a sequence of cell shapes and subsequently computes cellular traction forces, thus avoiding the requirement of a specialized fiducial-marked deformable substrate or force-free reference image. Rather, this technique drastically simplifies the overall experimental methodology, imaging, and analysis needed to conduct cell contractility measurements. We demonstrate that DeepMorphoTM quantitatively matches conventional TFM results, while offering stability against the biological variability in cell contractility for a given cell shape. Without high-frequency noise in the inferred displacement, DeepMorphoTM also resolves the ill-posedness of traction computation, increasing the consistency and accuracy of traction analysis. We demonstrate the accurate extrapolation across several cell types and substrate materials, suggesting robustness of the methodology. Accordingly, we present DeepMorphoTM as a capable yet simpler alternative to conventional TFM for characterizing cellular contractility in 2D.
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