DNA甲基化
甲基化
表观遗传学
有氧运动
医学
基因表达
内科学
内分泌学
体育锻炼
有氧能力
生理学
基因
生物
遗传学
作者
Koji Nakajima,Michiko Takeoka,Masayuki Mori,Satomi Hashimoto,Akihiro Sakurai,Hiroshi Nosé,Kayoko Higuchi,Naoki Itano,Masaaki Shiohara,Tae-Woong Oh,Shun’ichiro Taniguchi
出处
期刊:International Journal of Sports Medicine
[Georg Thieme Verlag KG]
日期:2010-03-03
卷期号:31 (09): 671-675
被引量:152
标识
DOI:10.1055/s-0029-1246140
摘要
Chronic moderate exercise has been reported to reduce pro-inflammatory cytokines. To analyze the molecular mechanisms by which training exerts these effects, the epigenetic influences of age and exercise on the ASC gene, which is responsible for IL-1β and IL-18 secretion, were investigated by ASC gene methylation. Further, the relationship between carcinogenesis and exercise, and methylation of the p15 tumor suppressive gene was also analyzed. High-intensity interval walking exercise, consisting of 3 min low-intensity walking at 40% of peak aerobic capacity followed by a 3 min high-intensity walking period above 70% of peak aerobic capacity, was continued for 6 months. Peripheral blood DNA extracts from young control (n=34), older control (n=153), and older exercise (n=230) groups were then analyzed by pyrosequencing for DNA methylation. Methylation of ASC decreased significantly with age (young control vs. older control, p<0.01), which is indicative of an age-dependent increase in ASC expression. Compared to the older control group, the degree of ASC methylation was higher in the older exercise group (older control vs. older exercise: p<0.01), and presumably lower ASC expression. Neither exercise nor age affected the methylation of the p15. In summary, chronic moderate exercise appears to attenuate the age-dependent decrease in ASC methylation, implying suppression of excess pro-inflammatory cytokines through reduction of ASC expression.
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