阿洛西林
适体
化学
检出限
胶体金
色谱法
吸光度
线性范围
自来水
分析化学(期刊)
核化学
纳米颗粒
材料科学
纳米技术
工程类
环境工程
生物
细菌
遗传学
铜绿假单胞菌
哌拉西林
作者
S. Y. Xiao,Jiping Lu,Liang Sun,Shengli An
标识
DOI:10.1016/j.saa.2022.120924
摘要
A new colorimetric biosensor for specific detection of azlocillin was developed by using DNA aptamer as recognition element and unmodified gold nanoparticles (AuNPs) as colorimetric indicator. In the absence of azlocillin, the AuNPs were protected by the aptamer and stabilized at high NaCl concentrations, displaying a red solution. In the presence of azlocillin, the aptamer reacts specifically with azlocillin, resulting in the aggregation of AuNPs and an apparent red to blue color change. The characteristic change can be easily observed by the naked eye and quantitatively detected by an ultraviolet-visible (UV-Vis) spectrometer. Under the optimal conditions, the absorbance variation at 522 nm (ΔA522) of AuNPs changed proportionally with increasing concentration of azlocillin, which exhibited a linear relationship in the concentration range of 50 nM to 500 nM, with a detection limit of 11.6 nM. Furthermore, the aptasensor was successfully used to detect azlocillin in milk and tap water samples, with recoveries ranging from 97.64% to 102.21% and a relative standard deviation (RSD) less than 3.81%.
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