代谢组学
嘧啶代谢
嘌呤
生物化学
代谢途径
嘌呤代谢
蛋白质组学
新陈代谢
低牛磺酸
化学
生物
药理学
牛磺酸
生物信息学
酶
氨基酸
基因
作者
Zhao Dong-sheng,Li-Long Jiang,Lingli Wang,Zi-Tian Wu,Zhuoqing Li,Wei Shi,Ping Li,Yan Jiang,Hui‐Jun Li
标识
DOI:10.1021/acs.chemrestox.8b00066
摘要
Previous studies have shown that Dioscorea bulbifera rhizome (DBR) can induce hepatotoxicity in clinical practice. However, its underlying mechanisms remain largely unexplored. In the present study, we investigated the global effect of DBR exposure on the proteomic and metabolomic profiles in rats over a 12-week administration using an integrated proteomics and metabolomics approach. The abundance of 1366 proteins and 58 metabolites in the liver of rats after subchronic exposure to DBR was dose-dependently altered. The results indicated that DBR mainly damaged hepatic cells through the aberrant regulation of multiple systems mainly including purine metabolism, pyrimidine metabolism, taurine and hypotaurine metabolism, and bile acid metabolism. Notably, the deregulated proteins including Pnp, Dpyd, Upp1, and Tymp and the differential metabolites including uridine, uracil, cytidine, thymine, adenine, adenosine, adenosine 3'-monophosphate, and deoxycytidine were well correlated to purine and pyrimidine metabolism, which might be novel pathways involved in metabolic abnormalities in rats with DBR-induced liver damage. Collectively, these findings not only contributed to understanding the mechanisms underlying the hepatotoxicity of DBR, but also illustrated the power of integrated proteomics and metabolomics approaches to improve the identification of metabolic pathways and biomarkers indicative of herb-induced liver injury.
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