免疫组织化学
免疫印迹
小桶
免疫系统
生物
基因
癌症研究
舌头
图谱
基因表达
污渍
细胞
渗透(HVAC)
病理
肿瘤科
转录组
医学
免疫学
蛋白质表达
遗传学
物理
热力学
作者
Zhuoyue Yao,Jing Chen,Li Wang,Liyu Cao
标识
DOI:10.1016/j.archoralbio.2024.106000
摘要
To explore the expression of HAUS6 in squamous cell carcinoma of the tongue (TSCC) and its relationship with the clinicopathological features of patients, and to further provide new ideas and therapeutic targets for curing TSCC. Design The Cancer Genome Atlas (TCGA) database was used to screen for differentially expressed genes (DEGs) between TSCC and normal tissues and survival analysis. DEGs of HAUS6 were screened and analyzed for GO, KEGG and GSEA enrichment. Exploring the correlation of HAUS6 with immune cell infiltration and immune checkpoint-related genes. The expression of HAUS6 in tumor and paraneoplastic tissues was confirmed by immunohistochemistry and Western Blot. Analysis of the TCGA database results showed that expression of HAUS6 mRNA was significantly enhanced and correlated with overall survival (OS, p<0.05) in TSCC. HAUS6 expression correlated with the level of immune cell infiltration and immune checkpoint-related genes. Immunohistochemistry and Western Blot confirmed that the expression level of HAUS6 protein was significantly higher in tumor tissues than in paraneoplastic tissues, and that tumor size and hypo-differentiation were higher in the HAUS6 high expression group than in the low expression group in TSCC (p<0.05). In conclusion, these analyses suggest that HAUS6 can act as an independent predictor of prognosis (p<0.05) and high HAUS6 expression is strongly associated with poor prognosis.
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