生物
秀丽隐杆线虫
长寿
转录组
遗传学
表型
基因
突变体
上位性
模式生物
计算生物学
基因表达
作者
Seokjin Ham,Sieun S. Kim,Sang‐Soon Park,Hyuck-Cheol Kwon,Seokjun G. Ha,Yunkyu Bae,Gee‐Yoon Lee,Seung‐Jae Lee
出处
期刊:Aging Cell
[Wiley]
日期:2024-03-26
卷期号:23 (7)
被引量:1
摘要
Abstract Classical genetic analysis is invaluable for understanding the genetic interactions underlying specific phenotypes, but requires laborious and subjective experiments to characterize polygenic and quantitative traits. Contrarily, transcriptomic analysis enables the simultaneous and objective identification of multiple genes whose expression changes are associated with specific phenotypes. Here, we conducted transcriptomic analysis of genes crucial for longevity using datasets with daf‐2 /insulin/IGF‐1 receptor mutant Caenorhabditis elegans . Our analysis unraveled multiple epistatic relationships at the transcriptomic level, in addition to verifying genetically established interactions. Our combinatorial analysis also revealed transcriptomic changes associated with longevity conferred by daf‐2 mutations. In particular, we demonstrated that the extent of lifespan changes caused by various mutant alleles of the longevity transcription factor daf‐16 / FOXO matched their effects on transcriptomic changes in daf‐2 mutants. We identified specific aging‐regulating signaling pathways and subsets of structural and functional RNA elements altered by different genes in daf‐2 mutants. Lastly, we elucidated the functional cooperation between several longevity regulators, based on the combination of transcriptomic and molecular genetic analysis. These data suggest that different biological processes coordinately exert their effects on longevity in biological networks. Together our work demonstrates the utility of transcriptomic dissection analysis for identifying important genetic interactions for physiological processes, including aging and longevity.
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