氧化磷酸化
氧化损伤
DNA
生物
DNA损伤
细胞生物学
大豆疫霉
核糖核酸
微生物学
疫霉菌
遗传学
氧化应激
基因
病菌
生物化学
植物
作者
Fan Zhang,Borui Zhang,Tongshan Cui,Shanshan Chen,Can Zhang,Zhiwen Wang,Xili Liu
出处
期刊:PLOS Pathogens
[Public Library of Science]
日期:2024-09-23
卷期号:20 (9): e1012553-e1012553
标识
DOI:10.1371/journal.ppat.1012553
摘要
N6-methyladenosine (m6A), a vital post-transcriptional regulator, is among the most prevalent RNA modifications in eukaryotes. Nevertheless, the biological functions of m6A in oomycetes remain poorly understood. Here, we showed that the PsMTA1 and PsMTA2 genes are orthologs of human METTL4, while the PsMET16 gene is an ortholog of human METTL16. These genes are implicated in m6A modification and play a critical role in the production of sporangia and oospores, the release of zoospores, and the virulence of Phytophthora sojae. In P. sojae, m6A modifications are predominantly enriched in the coding sequence and the 3' untranslated region. Notably, the PsMTA1 knockout mutant exhibited reduced virulence, attributed to impaired tolerance to host defense-generated ROS stress. Mechanistically, PsMTA1-mediated m6A modification positively regulates the mRNA lifespan of DNA damage response (DDR) genes in reaction to plant ROS stress during infection. Consequently, the mRNA abundance of the DDR gene PsRCC1 was reduced in the single m6A site mutant ΔRCC1/RCC1A2961C, resulting in compromised DNA damage repair and reduced ROS adaptation-associated virulence in P. sojae. Overall, these results indicate that m6A-mediated RNA metabolism is associated with the development and pathogenicity of P. sojae, underscoring the roles of epigenetic markers in the adaptive flexibility of Phytophthora during infection.
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