CYP8B1 Catalyzes 12alpha-hydroxylation ofC27Bile Acid: In Vitro Conversion ofDihydroxycoprostanic Acid into Trihydroxycoprostanic Acid

CYP8B1 is the unique P450 enzyme with sterol 12-oxidation activity, playing an exclusive role in 12α-hydroxylating intermediates along the bile acid (BA) synthesis pathway. Despite the long history of BA metabolism studies, it is unclear whether CYP8B1 catalyzes 12α-hydroxylation of C