亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Interrogation of the Substrate Profile and Catalytic Properties of the Phosphotriesterase from Sphingobium sp. Strain TCM1: An Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants and Plasticizers

化学 水解 增塑剂 有机化学 神经毒剂 氟磷酸二异丙酯 磷酸三苯酯 阻燃剂 乙酰胆碱酯酶
作者
Dao Feng Xiang,Andrew N. Bigley,Zhongjie Ren,Haoran Xue,Kenneth G. Hull,Daniel Romo,Frank M. Raushel
出处
期刊:Biochemistry [American Chemical Society]
卷期号:54 (51): 7539-7549 被引量:32
标识
DOI:10.1021/acs.biochem.5b01144
摘要

The most familiar organophosphorus compounds are the neurotoxic insecticides and nerve agents. A related group of organophosphorus compounds, the phosphotriester plasticizers and flame retardants, has recently become widely used. Unlike the neurotoxic phosphotriesters, the plasticizers and flame retardants lack an easily hydrolyzable bond. While the hydrolysis of the neurotoxic organophosphates by phosphotriesterase enzymes is well-known, the lack of a labile bond in the flame retardants and plasticizers renders them inert to typical phosphotriesterases. A phosphotriesterase from Sphingobium sp. strain TCM1 (Sb-PTE) has recently been reported to catalyze the hydrolysis of organophosphorus flame retardants. This enzyme has now been expressed in Escherichia coli, and the activity with a wide variety of organophosphorus substrates has been characterized and compared to the activity of the well-known phosphotriesterase from Pseudomonas diminuta (Pd-PTE). Structure prediction suggests that Sb-PTE has a β-propeller fold, and homology modeling has identified a potential mononuclear manganese binding site. Sb-PTE exhibits catalytic activity against typical phosphotriesterase substrates such as paraoxon, but unlike Pd-PTE, Sb-PTE is also able to effectively hydrolyze flame retardants, plasticizers, and industrial solvents. Sb-PTE can hydrolyze both phosphorus–oxygen bonds and phosphorus–sulfur bonds, but not phosphorus–nitrogen bonds. The best substrate for Sb-PTE is the flame retardant triphenyl phosphate with a kcat/Km of 1.7 × 106 M–1 s–1. Quite remarkably, Sb-PTE is also able to hydrolyze phosphotriesters with simple alcohol leaving groups such as tributyl phosphate (kcat/Km = 40 M–1 s–1), suggesting that this enzyme could be useful for the bioremediation of a wide variety of organophosphorus compounds.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
ssherry完成签到,获得积分10
4秒前
陶醉巧凡发布了新的文献求助10
10秒前
13秒前
Marlowe7发布了新的文献求助10
17秒前
赘婿应助忐忑的棉花糖采纳,获得10
22秒前
Norcae完成签到 ,获得积分10
26秒前
海边的曼彻斯特完成签到 ,获得积分10
31秒前
31秒前
陶醉巧凡发布了新的文献求助10
34秒前
36秒前
51秒前
Marlowe7完成签到,获得积分10
54秒前
悦耳碧萱发布了新的文献求助10
58秒前
1分钟前
1分钟前
踏实凡儿完成签到 ,获得积分10
1分钟前
爆米花应助科研通管家采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
山猪吃细糠完成签到 ,获得积分10
1分钟前
xbb0905发布了新的文献求助10
1分钟前
Haoru完成签到,获得积分10
1分钟前
1分钟前
Jasper应助忐忑的棉花糖采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
1分钟前
2分钟前
科研通AI6.2应助Amor采纳,获得10
2分钟前
2分钟前
情怀应助自然映梦采纳,获得10
2分钟前
2分钟前
舒服的如蓉完成签到,获得积分10
2分钟前
涵de暴躁小地雷完成签到,获得积分10
2分钟前
2分钟前
九灶完成签到 ,获得积分10
2分钟前
bji完成签到,获得积分10
2分钟前
高分求助中
(应助此贴封号)【重要!!请各用户(尤其是新用户)详细阅读】【科研通的精品贴汇总】 10000
Modern Epidemiology, Fourth Edition 5000
Handbook of pharmaceutical excipients, Ninth edition 5000
Digital Twins of Advanced Materials Processing 2000
Weaponeering, Fourth Edition – Two Volume SET 2000
Polymorphism and polytypism in crystals 1000
Social Cognition: Understanding People and Events 800
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 纳米技术 有机化学 物理 生物化学 化学工程 计算机科学 复合材料 内科学 催化作用 光电子学 物理化学 电极 冶金 遗传学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 6027671
求助须知:如何正确求助?哪些是违规求助? 7679335
关于积分的说明 16185657
捐赠科研通 5175123
什么是DOI,文献DOI怎么找? 2769225
邀请新用户注册赠送积分活动 1752618
关于科研通互助平台的介绍 1638422