细菌
二甲戊灵
杀虫剂
微生物
生物
微生物学
分离(微生物学)
芽孢杆菌(形态)
假单胞菌
生物技术
化学控制
生态学
农学
遗传学
作者
Ya-Peng Peng,Liang Ma,Ying Huang,Ming‐He Mo,Jian-Jin Liu,Tong Liu
出处
期刊:Fems Microbiology Letters
[Oxford University Press]
日期:2024-01-01
卷期号:371
标识
DOI:10.1093/femsle/fnae052
摘要
The extensive use of chemical pesticides, such as herbicides, has resulted in significant environmental pollution. Microbial degradation represents a crucial approach for managing this pesticide-associated pollution, with enrichment culturing serving as a method for isolating pesticide-degrading microorganisms. However, the efficiency of this strategy is limited, often yielding only a few isolated strains. In this study, a new mineral salt medium (MSM) was developed, and a high-throughput method was used for screening pendimethalin-degrading bacteria by measuring the bacterial growth in the MSM. The utilization of this method resulted in the isolation of 56 pendimethalin-degrading bacteria from approximately 2000 bacterial strains, including 37 Bacillus spp., 10 Alcaligenes spp., 5 Pseudomonas spp., and other 4 strains identified for the first time as pendimethalin-degrading strains. This method may hold promise not only for isolating bacterial strains capable of degrading other pesticides but also for facilitating the utilization of the substantial bacterial strains stored in bacterial banks.
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