Single Extracellular Vesicles Physical and biochemical characterization for single extracellular vesicles (EVs) has been gaining more attention for disease diagnosis. With the development of micromachining technologies, microfluidic chips can analyze EVs with precise manipulation, low cost and high portability. In the foreseeable future, the combination of EVs characterization with microfluidics and gradually individualizing them will be a meaningful direction. These considerations are reviewed by Daxiang Cui, Hossam Haick, Ning Tang, and co-workers, in article 2202437.