毒性
鼻腔给药
化学
细胞凋亡
活力测定
体内
流式细胞术
细胞毒性
体外
吸入
分子生物学
药理学
生物物理学
生物化学
生物
解剖
有机化学
生物技术
作者
Jiayu Huang,Guangheng Dong,Miaoting Liang,Xuecheng Wu,Mingjian Xian,Yunsong An,Jiandong Zhan,Lei Xu,Jindong Xu,Weimin Sun,Shaohua Chen,Chengyu Chen,Tao Liu
出处
期刊:Chemosphere
[Elsevier]
日期:2022-11-01
卷期号:307: 136093-136093
被引量:22
标识
DOI:10.1016/j.chemosphere.2022.136093
摘要
Micro (nano)plastics (MNPs) have become emerging environmental contaminants, yet their toxicity and systemic effects via intranasal exposure remain unclear. This study investigated the in vitro toxicity of thirteen polystyrene MNPs with different surface functionalization (carboxylic (C-PS), amino (A-PS), and bare (PS)) and sizes (20-2000 nm) on human nasal epithelial cells (HNEpCs) at 10-1250 μg/mL as well as their in vivo toxicity to rats via intranasal administration at 125 μg/mL. The in vitro study showed that PS20, PS50, A-PS50, PS500, and A-PS500 significantly inhibited cell viability, which was dependent on particle concentration. A-PS induced higher cytotoxicity than C-PS and PS, and most MNPs inhibited cell proliferation after 24-h. Flow cytometry analysis suggested that PS induced cell apoptosis, while A-PS caused cell necrosis. MNPs were phagocytosed by HNEpCs and entered nucleus. The in vivo study showed that MNPs inhibited dietary behaviors of rats. Histological analysis indicated that PS20, PS200, and A-PS50 thinned out nasal mucosa. Immunohistochemical analysis revealed that exposure to PS20, PS200, and A-PS50 enhanced expression of transient receptor potential cation channel subfamily M (melastatin) member 8 (TRPM8). Systemic effects including hepatocyte cytoplasmic vacuolation and renal tubule dilatation were observed. The results suggested that nasal inhalation of MNPs may disturb energy metabolism and damage upper respiratory tract, liver, and kidneys.
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