Ethyl Acetate Extracts of Two Artemisia Species: Analyses of Phenolic Profile and Anticancer Activities Against SW-480 Colon Cancer Cells

Because Artemisia vulgaris L. and Artemisia alba Turra are traditional medicinal plants used for the treatment of different diseases, we evaluated the cytotoxic/apoptotic activity of ethyl acetate extracts from these natural products against human colon cancer cells SW-480. The extracts contained a large amount of the total polyphenols and flavonoids. The phenolic profile showed the presence of phenolic acids (gallic, p-coumaric, vanillic, and ferulic acids) and flavonoids (rutin, myricetin, luteolin, quercetin, and apigenin). 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay indicated that antiproliferative activities of both A. vulgaris and A. alba extracts increased with the extension of time exposure, with decreasing IC 50 values. Mitomycin C (MMC) alone had antiproliferative activity, but in combination with plant extracts caused stronger effect with lower IC 50 values. Flow cytometry analyses showed that A. alba extract induced higher percentage of SW-480 cells in the early stage of apoptosis (33.5 ± 1.6 vs 0.7 ± 0.1, P < 0.05), whereas the A. vulgaris extract significantly increased the percentage of cells in necrosis (82.4 ± 5.0 vs 53.9 ± 2.3, P < 0.05). In conclusion, A. alba extract can be considered a potential source of bioactive components with anticancer activity or be used as a dietary food supplement or supplement to chemotherapy due to its synergistic effect with the MMC.