生物
东方果蝇
RNA干扰
基因敲除
小RNA
基因沉默
小RNA
阿尔戈瑙特
Piwi相互作用RNA
卵巢
基因
RNA沉默
小干扰RNA
遗传学
有害生物分析
核糖核酸
植物
铁杉科
作者
Ruining Yang,Qiang Zhang,Jiaqi Fan,Yong Yue,Er‐Hu Chen,Guangjie Yuan,Wei Dou,Jinjun Wang
摘要
Abstract BACKGROUND With the development of rapid resistance, new modes of action for pesticides are needed for insect control, such as RNAi‐based biopesticides targeting essential genes. To explore the function of Argonaute‐1 ( Ago‐1 ) and potential miRNAs in ovarian development of Bactrocera dorsalis , an important agricultural pest, and to develop a novel control strategy for the pest, BdAgo‐1 was first identified in B. dorsalis . RESULTS Spatiotemporal expression analysis indicated that BdAgo‐1 had a relatively high transcriptional level in the ovarian tissues of adult female B. dorsalis during the sexual maturation period. RNA interference (RNAi) experiment showed that BdAgo‐1 knockdown significantly decreased the expression levels of ovarian development‐related genes and delayed ovarian development. Although RNAi‐mediated silencing of Ago‐1 led to a reduced ovary surface area, a subsequent oviposition assay revealed that the influence was minimal over a longer time period. Small RNA libraries were constructed and sequenced from different ovarian developmental stages of B. dorsalis adults. Among 161 identified miRNAs, 84 miRNAs were differentially expressed during the three developmental stages of the B. dorsalis ovary. BdAgo‐1 silencing caused significant down‐regulation of seven differentially expressed miRNAs (DEMs) showing relatively high expression levels (>1000 TPM (Transcripts per kilobase of exon model per million mapped reads)). The expression patterns of these seven core DEMs and their putative target genes were analyzed in the ovaries of B. dorsalis . CONCLUSION The results indicate that Ago‐1 and Ago‐1 ‐dependent miRNAs are indispensable for normal ovarian development in B. dorsalis and help identify miRNA targets useful for control of this pest.
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