再现性
代谢物
代谢组学
金黄色葡萄球菌
猝灭(荧光)
色谱法
产量(工程)
过滤(数学)
化学
生物
生物化学
细菌
材料科学
数学
物理
统计
量子力学
冶金
荧光
遗传学
作者
Xin Wu,Hailong Yu,Zhao‐Yong Ba,Jing‐Yu Chen,Hong‐Gang Sun,Bei‐Zhong Han
标识
DOI:10.1002/biot.200900038
摘要
To select an appropriate sampling method for comparison of metabolite profiles between planktonic and biofilm Staphylococcus aureus using NMR techniques, we evaluated three methods: quenching-centrifugation (QC), filtration-quenching (FQ) and filtration-quenching-lyophilization (FQL). We found differences in metabolite loss, yield, reproducibility and metabolite profile. QC caused severe metabolite leakage and possible decomposition of nucleotides. FQ achieved high yields and reproducibility, although it had the disadvantages of long filtration and rinse times before quenching. FQL resulted in a loss of a few metabolites and a lower yield due to lyophilization. Although the biomarkers discovered by each method were nearly the same and seemed insensitive to technical variances, we conclude that FQ is the most appropriate sampling method because of its high yield and reproducibility.
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