多糖
化学
发酵
低聚糖
食品科学
大小排阻色谱法
双歧杆菌
细菌
色谱法
生物化学
乳酸菌
生物
酶
遗传学
作者
Ai Ling Ho,Ondřej Kosík,Alison Lovegrove,Dimitris Charalampopoulos,Robert A. Rastall
标识
DOI:10.1016/j.carbpol.2017.08.077
摘要
Xylo-oligosaccharides and xylo-polysaccharides (XOS, XPS) produced by autohydrolysis of the fibre from oil palm empty fruit bunches (OPEFB) were purified using gel filtration chromatography to separate the XOS and XPS from the crude autohydrolysis liquor. Six mixed fractions of refined XOS and XPS with average degree of polymerisation (avDP) of 4–64 were obtained. These were characterised in terms of their composition and size by HPLC, MALDI-ToF-MS (selected fractions) and carbohydrate gel electrophoresis (PACE). They were assessed in batch culture fermentations using faecal inocula to determine their ability to modulate the human faecal microbiota in vitro by measuring the bacterial growth, organic acid production and the XOS assimilation profile. The gut microbiota was able to utilise all the substrates and there was a link between the avDP with the fermentation properties. In general, XOS/XPS preparations of lower avDP promote better Bifidobacterium growth and organic acid production.
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