A facile and simple polyaniline-poly(ethylene oxide) based glucose biosensor

In this work, the fabrication and performance of a simple and efficient glucose biosensor based on chemically synthesized polyaniline-poly(ethylene oxide) nanocomposite (PAni-PEO) have been demonstrated. The nanocomposite was synthesized through rapid-mixing polymerization and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR). The nanocomposite and glucose oxidase (GOx) solutions were placed on a platinum electrode by drop-casting, to produce a glucose biosensor that was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronoamperometry. Under optimal conditions, the biosensor showed a linear range from 1 to 10 mM (R2 = 0.990) with a high sensitivity of 16.04 μA mM−1 cm−2 and a detection limit of 0.82 mM. The interference from ascorbic acid (AA) and uric acid (UA) was also investigated. The results indicated that the biosensor exhibited a satisfactory linear range, sensibility, and selectivity for use as a glucose biosensor, considering the normal range of approximately 4–6 mM of glucose concentration in human blood.