生物
锌指
转录因子
抄写(语言学)
功能(生物学)
核糖核酸
细胞生物学
转录调控
计算生物学
遗传学
基因
语言学
哲学
作者
Syed Nabeel‐Shah,Shuye Pu,James Burns,Ulrich Braunschweig,Nujhat Ahmed,Giovanni L. Burke,Hyunmin Lee,Ernest Radovani,Guoqing Zhong,Hua Tang,Edyta Marcon,Zhaolei Zhang,Timothy R. Hughes,Benjamin J. Blencowe,Jack Greenblatt
标识
DOI:10.1016/j.molcel.2024.08.037
摘要
Cys2-His2 zinc-finger proteins (C2H2-ZNFs) constitute the largest class of DNA-binding transcription factors (TFs) yet remain largely uncharacterized. Although certain family members, e.g., GTF3A, have been shown to bind both DNA and RNA, the extent to which C2H2-ZNFs interact with-and regulate-RNA-associated processes is not known. Using UV crosslinking and immunoprecipitation (CLIP), we observe that 148 of 150 analyzed C2H2-ZNFs bind directly to RNA in human cells. By integrating CLIP sequencing (CLIP-seq) RNA-binding maps for 50 of these C2H2-ZNFs with data from chromatin immunoprecipitation sequencing (ChIP-seq), protein-protein interaction assays, and transcriptome profiling experiments, we observe that the RNA-binding profiles of C2H2-ZNFs are generally distinct from their DNA-binding preferences and that they regulate a variety of post-transcriptional processes, including pre-mRNA splicing, cleavage and polyadenylation, and m
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