Pollutant accumulation characteristics of substrates and mechanism of microbial action in different ecological ditches

Ecological ditches serve as one of the important measures for the concentrated infiltration of stormwater in the construction process of sponge cities. Prolonged concentrated infiltration of stormwater can lead to the accumulation of pollutants and pollution risks in the substrate of ecological ditches. In this study, two different substrate ecological ditches were constructed, namely, a combined substrate ecological ditch with zeolite + ceramsite (EA), and a biological substrate ecological ditch (EB). The accumulation characteristics of pollutants and microbial mechanisms in the ecological ditches were evaluated by simulating the cumulative rainfall test for three years. Results showed that there was serious total nitrogen (TN) contamination in both EA and EB substrates, with mean values of 718.6 and 802.7 mg/kg, respectively. The average contents of potentially toxic elements (Cu, Pb, and Zn) in EA and EB substrates were higher than the background values of soil elements in China, and Cu posed considerable ecological risks to the environment. Proteobacteria was the dominant phylum and accounted for 32.85-41.33% in two ditches; the total number of denitrifying functional bacterial genera in the EB increased by 1.53% than EA, which proved that the biological substrate was more favourable for the growth of denitrifying microorganisms. The accumulation characteristics of pollutants and microbial mechanisms in the ecological ditches with different substrates above provide a reference for extending the service life of ecological ditch facilities.