Preparation of β‐cyclodextrin covalent organic framework‐immobilized poly(glycidyl methacrylate) nanoparticle‐coated open tubular capillary electrochromatography column for chiral separation

A new enantioselective open‐tubular capillary electrochromatography was developed employing poly(glycidyl methacrylate) nanoparticles/β‐cyclodextrin covalent organic frameworks chemically immobilized on the inner wall of the capillary as a stationary phase. A pretreated silica‐fused capillary reacted with 3‐aminopropyl‐trimethoxysilane followed by poly(glycidyl methacrylate) nanoparticles and β‐cyclodextrin covalent organic frameworks through a ring‐opening reaction. The resulting coating layer on the capillary was characterized by scanning electron microscopy and Fourier transform infrared spectroscopy. The electroosmotic flow was studied to evaluate the variation of the immobilized columns. The chiral separation performance of the fabricated capillary columns was validated by the analysis of the four racemic proton pump inhibitors including lansoprazole, pantoprazole, tenatoprazole, and omeprazole. The influences of bonding concentration, bonding time, bonding temperature, buffer type and concentration, buffer pH, and applied voltage on the enantioseparation of four proton pump inhibitors were investigated. Good enantioseparation efficiencies were achieved for all enantiomers. In the optimum conditions, the enantiomers of four proton pump inhibitors were fully resolved within 10 min with high resolutions of 9.5–13.9. The column‐to‐column and inter‐ to intra‐day repeatability of the fabricated capillary columns through relative standard deviation were found better than 9.54%, exhibiting satisfactory stability and repeatability of the fabricated capillary columns.