岩藻糖基转移酶
乳糖
巨芽孢杆菌
大肠杆菌
拉伤
酶
生物化学
发酵
细菌
化学
生产过剩
生物
食品科学
微生物学
基因
遗传学
解剖
作者
Jun-Min Lee,Bu-Soo Park,Min‐Kyu Oh
标识
DOI:10.1016/j.enzmictec.2023.110232
摘要
2'-Fucosyllactose (2'-FL) is a major oligosaccharide found in human breast milk. It is produced from GDP-L-fucose and D-lactose by ⍺1,2-fucosyltransferase (⍺1,2-fucT), but the enzyme has been identified mostly in pathogens. In this study, an ⍺1,2-fucT was isolated from a Generally Recognized as Safe (GRAS) Bacillus megaterium strain. The enzyme was successfully expressed in metabolically-engineered Escherichia coli. Furthermore, replacement of non-conserved amino acid residues with conserved ones in the protein led to an increase in the rate of 2'-FL production. As a result, fed-batch fermentation of E. coli produced 30 g/L of 2'-FL from glucose and lactose. Thus, the overproduction of 2'-FL using a novel enzyme from a GRAS bacteria strain was successfully demonstrated.
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