This study aimed to develop paper packaging from rice straw fibers with its function as antibacterial activity obtained from pomelo ( Citrus maxima ) peels. Paper with ca. 106.42 g/m 2 basis weight, 0.34 mm thickness, 34.15 % brightness and 78.7 N.m/g tensile index was prepared from rice straw fibers and pulps. Bioactive compounds of crude pomelo peels extracted using maceration technique with 75% (v/v) ethanol were coated onto rice straw papers at concentration of 10, 15 and 20 % (w/v). The coated papers had similar thickness, and slightly lower tensile index and brightness as compared to those of control papers. Scanning electron microscopy confirmed the changes of surface and cross-section structures of coated and uncoated papers. The rice straw coated papers had relatively greater barrier property to prevent water absorption. In addition, the coated papers with pomelo peel extract showed significant antibacterial activity against food pathogenic bacteria, including Staphylococcus aureus , Bacillus cereus and Escherichia coli . This study reveals the benefits of natural by-products as a potential material for active packaging.