In silico interactome analysis of cross-link repair proteins (RPA32b and RPA2) in Arabidopsis thaliana

DNA molecule is found in every cell in a living organism and function as an instruction manual for growth, development, as well as any other processes that happen. As the cell divide, it needs to copy its DNA. During the copying, an error can occur, which can lead to a disease. However, there are different error repair mechanisms that are searching for an error, and repairing it. This study focused on 2 proteins involved in DNA repair mechanism, RPA32B and RPA2 from Arabidopsis thaliana. Throughout multiple in silico approached, multiple sequence alignment, phylogenetic tree construction, 3D visualisation, combination and interaction, we confirmed that the RPA2 and RPA 32b are involved in DNA replication, meiotic recombination and DNA repair mechanism.