Andrographolide Inhibits Inflammatory Cytokines Secretion in LPS-Stimulated RAW264.7 Cells through Suppression of NF-κB/MAPK Signaling Pathway

穿心莲内酯 MAPK/ERK通路 NF-κB 信号转导 化学 NFKB1型 分泌物 炎症 细胞生物学 生物 生物化学 免疫学 转录因子 基因
作者
Li Yu,Shengnan He,Jishun Tang,Nana Ding,Xiaoyan Chu,Lianping Cheng,Xuedong Ding,Ting Liang,Shibin Feng,Sajid Ur Rahman,Xichun Wang,Jinjie Wu
出处
期刊:Evidence-based Complementary and Alternative Medicine [Hindawi Limited]
卷期号:2017 (1) 被引量:62
标识
DOI:10.1155/2017/8248142
摘要

Andrographolide, the main active component extracted from Andrographis paniculata (Burm.f.) Wall. ex Nees, exerts anti‐inflammatory effects; however, the principal molecular mechanisms remain unclear. The objective of this study was to investigate the molecular mechanisms of Andrographolide in modifying lipopolysaccharide‐ (LPS‐) induced signaling pathway in RAW264.7 cells. An in vitro model of inflammation was induced by LPS in mouse RAW264.7 cells in the presence of Andrographolide. The concentration and expression levels of proinflammatory cytokines were determined by an enzyme‐linked immunosorbent assay (ELISA) and quantitative real‐time polymerase chain reaction (qRT‐PCR), respectively. The nuclear level of NF‐ κ B was measured by an electrophoretic mobility shift assay (EMSA). The expression levels of NF‐ κ B, p38, ERK, and JNK were determined by western blot. Andrographolide dose‐dependently inhibited the release and mRNA expression of TNF‐ α , IL‐6, and IL‐1 β in LPS‐stimulated RAW264.7 cells. The nuclear level of p65 protein was decreased in Andrographolide treatment group. Western blot analysis showed that Andrographolide suppressed LPS‐induced NF‐ κ B activation and the phosphorylation of IkBa, ERK1/2, JNK, and p38. These results suggest that Andrographolide exerts an anti‐inflammatory effect by inhibiting the activation of NF‐ κ B/MAPK signaling pathway and the induction of proinflammatory cytokines.
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