Novel Anthocyanin-Based Colorimetric Assay for the Rapid, Sensitive, and Quantitative Detection of Helicobacter pylori

化学 红卷心菜 检出限 快速尿素酶试验 色谱法 幽门螺杆菌 pH指示剂 滤纸 花青素 RGB颜色模型 肉眼 比色法 食品科学 生物化学 人工智能 生物 计算机科学 物理化学 胃炎 遗传学
作者
Çağla Çelik,Gülten Can Sezgin,Umut Gorkem Kocabas,Şebnem Gürsoy,Nilay Ildız,Weihong Tan,İsmail Öçsoy
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:93 (15): 6246-6253 被引量:34
标识
DOI:10.1021/acs.analchem.1c00663
摘要

Several different diagnostic tests have been reported for rapid, sensitive, and economical detection of bacterial pathogens, but most lack widespread and practical use in the clinic. In this study, we used anthocyanins from red cabbage (Brassica oleracea) as a natural pH indicator and, for the first time, incorporated this agent into a simple, rapid, and economical colorimetric strategy for the detection of Helicobacter pylori (H. pylori) (RCE@test). We prepared two sets of RCE@test solutions (test 1 is purple, and test 2 is blue) in different forms, including liquid, adsorbed filter paper, and agar, and investigated the performance of each RCE@test as a function of the test volume, H. pylori concentration, and reaction time. To elucidate the effect of the pathophysiological environment on these RCE@tests, H. pylori in an artificial gastric fluid was also detected. The 10 and 1 CFU/mL H. pylori suspensions were detected in 15 min and 3 h, respectively, and the limit of detection was determined down to 1 CFU/mL. We experimentally demonstrated the advantages of the RCE@test for detection of H. pylori by comparing it to a commercially available rapid urease test, the "CLO test (Campylobacter-like organism test)". In addition to colorimetric detection by the naked eyes, RGB (Red Green Blue) and Delta-E analysis in image-processing software was run to quantitatively monitor changes of color in the RCE@test using a smartphone application. Finally, we propose that this test provides simple, effective, rapid, and inexpensive detection and that it can be easily implemented for clinical use.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Xu完成签到,获得积分10
1秒前
yuhe完成签到,获得积分20
3秒前
huan完成签到,获得积分10
3秒前
嘎嘎嘎发布了新的文献求助10
5秒前
星辰大海应助缓慢的灵枫采纳,获得10
5秒前
5秒前
orixero应助诸葛十八子采纳,获得10
6秒前
6秒前
Andrew应助一头小飞猪采纳,获得50
7秒前
9秒前
不胜寒发布了新的文献求助10
10秒前
CipherSage应助科研通管家采纳,获得10
12秒前
斯文败类应助科研通管家采纳,获得10
12秒前
Lucas应助科研通管家采纳,获得10
12秒前
Jasper应助科研通管家采纳,获得10
12秒前
12秒前
12秒前
12秒前
12秒前
青年才俊发布了新的文献求助10
13秒前
bmn完成签到,获得积分10
14秒前
hhahha完成签到,获得积分10
14秒前
火箭Lucky完成签到 ,获得积分10
14秒前
斯文败类应助yuhe采纳,获得10
14秒前
zzz駿完成签到,获得积分10
15秒前
阿rain完成签到,获得积分10
16秒前
kk应助jiazhou采纳,获得10
16秒前
qinsu完成签到,获得积分10
16秒前
yqcsysu发布了新的文献求助10
17秒前
18秒前
不胜寒完成签到,获得积分10
20秒前
唠叨的善若完成签到 ,获得积分10
20秒前
英俊的铭应助缓慢的灵枫采纳,获得10
21秒前
21秒前
kekeji发布了新的文献求助10
24秒前
小蘑菇应助jiang采纳,获得10
26秒前
无聊的老姆完成签到 ,获得积分10
27秒前
666完成签到,获得积分10
27秒前
遥感小虫发布了新的文献求助10
28秒前
隐形迎松完成签到 ,获得积分10
28秒前
高分求助中
Impact of Mitophagy-Related Genes on the Diagnosis and Development of Esophageal Squamous Cell Carcinoma via Single-Cell RNA-seq Analysis and Machine Learning Algorithms 2000
How to Create Beauty: De Lairesse on the Theory and Practice of Making Art 1000
Gerard de Lairesse : an artist between stage and studio 670
大平正芳: 「戦後保守」とは何か 550
2019第三届中国LNG储运技术交流大会论文集 500
Contributo alla conoscenza del bifenile e dei suoi derivati. Nota XV. Passaggio dal sistema bifenilico a quello fluorenico 500
Multiscale Thermo-Hydro-Mechanics of Frozen Soil: Numerical Frameworks and Constitutive Models 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 2998722
求助须知:如何正确求助?哪些是违规求助? 2659143
关于积分的说明 7199473
捐赠科研通 2294786
什么是DOI,文献DOI怎么找? 1216819
科研通“疑难数据库(出版商)”最低求助积分说明 593628
版权声明 592904