Comparative metabolomics and sensory evaluation of pineapple (Ananas comosus) reveal the importance of ripening stage compared to cultivar

The pineapple ripening stage is determined at the time of harvest and can be classified into the C0 to C4 stage based on its peel color. C1 and C4 pineapples are the most commonly exported products with high demand. Pineapple also has many cultivars with different tastes and appearances. However, there are limited studies regarding the metabolite and taste profiles of pineapple from different cultivars and ripening stages using a metabolomics approach. Therefore, the objective of this study was to investigate the metabolite and sensory profiles of pineapple from different cultivars and different ripening stages. Three cultivars of pineapple (Red Spanish, Smooth Cayenne, and Queen) and C1, C3, and C4 stages from Indonesia, Japan, the Philippines, and Taiwan, respectively, were analyzed by gas chromatography/mass spectrometry (GC/MS). The data obtained from GC/MS analysis were combined with the data obtained from sensory evaluation, which showed that sourness and firmness contributed to C1 fruits with metabolites correlated to isocitric acid+citric acid, malic acid, ascorbic acid, and quinic acid, while sweetness, juiciness, and high acceptability contributed to C4 fruits with metabolites that correlated were asparagine, serine, glycine, threonic acid, sucrose, and 5-hydroxytryptamine. GC/MS analysis using different pineapple cultivars also showed characteristic metabolites for each cultivar. Prediction of the sensory profile was performed by investigating the relative intensity of taste-related metabolites. This information can provide a basis for improving pineapple taste quality related to metabolites, depending on the cultivar and ripening stage.