适体
化学
检出限
分析物
胶体金
生物传感器
四环素
纳米颗粒
溴化物
色谱法
组合化学
比色法
纳米技术
无机化学
生物化学
材料科学
生物
抗生素
遗传学
作者
Lan He,Yanfang Luo,Wenting Zhi,Yuangen Wu,Pei Zhou
摘要
This paper proposes a sensing strategy which employs an aptamer, unmodified gold nanoparticles (AuNP), and hexadecyltrimethylammonium bromide (CTAB) to detect tetracycline (TET) in raw milk. The method is based on the colorimetric assay of aggregating AuNP. In the absence of TET, the CTAB and aptamer form a complex which allows the aggregation of AuNP. In the presence of TET, the TET aptamer is exhausted first due to the formation of aptamer-TET complexes, which prevents assembly of the CTAB–aptamer supramolecule, causing a colour change and no aggregation of AuNP. This mechanism for the detection of TET proved to be sensitive and convenient. The colorimetric assay has a detection limit of 122 nM TET. This sensor has great potential for the sensitive, colorimetric detection of a wide range of molecular analytes.
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