嗜铬细胞
肠内分泌细胞
生物
细胞生物学
受体
细胞分化
类有机物
内分泌学
生物化学
血清素
基因
内分泌系统
激素
作者
Gilles Dinsart,Morgane Leprovots,Anne Lefort,Frédérick Libert,Yannick Quesnel,Alex Veithen,Gilbert Vassart,Sandra Huysseune,Marc Parmentier,Marie‐Isabelle Garcia
标识
DOI:10.1038/s44319-023-00013-5
摘要
Abstract The gastrointestinal epithelium constitutes a chemosensory system for microbiota-derived metabolites such as short-chain fatty acids (SCFA). Here, we investigate the spatial distribution of Olfr78, one of the SCFA receptors, in the mouse intestine and study the transcriptome of colon enteroendocrine cells expressing Olfr78. The receptor is predominantly detected in the enterochromaffin and L subtypes in the proximal and distal colon, respectively. Using the Olfr78-GFP and VilCre/Olfr78flox transgenic mouse lines, we show that loss of epithelial Olfr78 results in impaired enterochromaffin cell differentiation, blocking cells in an undefined secretory lineage state. This is accompanied by a reduced defense response to bacteria in colon crypts and slight dysbiosis. Using organoid cultures, we further show that maintenance of enterochromaffin cells involves activation of the Olfr78 receptor via the SCFA ligand acetate. Taken together, our work provides evidence that Olfr78 contributes to colon homeostasis by promoting enterochromaffin cell differentiation.
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