The analysis of biosignals and medical images using artificial intelligence (AI) has become an important area of research in the field of biomedical engineering. This chapter provides an overview of the definition and classification of various types of biomedical signals and images, including electrical signals such as electrocardiogram, electroencephalogram, electromyogram, and magnetoencephalogram, as well as optical, thermal, mechanical, and acoustic signals. Each type of signal is described in detail, along with their corresponding medical applications. Furthermore, the chapter discusses the role of AI in the analysis of these signals and images, highlighting recent advancements and challenges in this field. The purpose of the chapter is to provide readers with a comprehensive understanding of the various types of biomedical signals and images, as well as the potential applications and challenges associated with the use of AI for their analysis.