分子内力
荧光
化学
联想(心理学)
活体细胞成像
计算生物学
纳米技术
组合化学
计算机科学
生物物理学
细胞
生物化学
立体化学
生物
材料科学
物理
光学
认识论
哲学
作者
Yoshiaki Hori,Kyohei Nakaki,Motoki Sato,Shin Mizukami,Kazuya Kikuchi
标识
DOI:10.1002/anie.201200867
摘要
Turn on the switch: Fluorogenic probes for protein labeling based on the photoactive yellow protein (PYP) tag were developed. The fluorescence of the probes is turned off by intramolecular association and switched on by the reversal of this interaction upon reaction with the PYP tag that is fused to the protein of interest (POI, see scheme). The rapid and specific labeling reaction enabled the imaging of cell-surface proteins without washing. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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