A label-free colorimetric aptasensor for rapid multiplex detection of leuco-malachite green and leuco-crystal violet

Leuco-malachite green (LMG) and leuco-crystal violet (LCV) are widespread co-pollutants in aquatic products that pose a severe threat to human health. Therefore, it is urgent and challenging to develop rapid multiplex detection of LMG and LCV. Herein, the bispecific aptamer (A5b) for LMG and LCV was characterized. Its dissociation constant (