磷酸戊糖途径
虾青素
生物化学
谷氨酸受体
柠檬酸循环
新陈代谢
化学
代谢途径
焊剂(冶金)
谷氨酸
生物
氨基酸
糖酵解
类胡萝卜素
受体
有机化学
作者
Chunyu Cai,Na Xu,Jiale Feng,Jiahua Zhang,Qianxi Zhao,Huimin Liu,Bo Nan,Xia Li,Yuhua Wang
标识
DOI:10.1016/j.biortech.2024.130834
摘要
Effective metabolic regulators play an essential role in regulating astaxanthin biosynthesis in Phaffia rhodozyma. In this study, it was found that 5 mM glutamate increased the astaxanthin yield and biomass of P. rhodozyma D3 to 22.34 mg/L and 6.12 g/L, which were 1.22 and 1.33 times higher than the control group, respectively. Meanwhile, glucose uptake was increased and the level of reactive oxygen species (ROS) was reduced with 5 mM glutamate. To further explore the interrelationship between glutamate and astaxanthin synthesis, the energy metabolism of P. rhodozyma D3 with and without glutamate was analysed. Glutamate promoted the Embden-Meyerhof-Parnas pathway (EMP) metabolic flux, modulated the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway (PPP), activated the ornithine cycle and purine metabolism, and provided more ATP and NADPH for astaxanthin accumulation. This study clarified the possible mechanism by which glutamate promoted astaxanthin accumulation in P. rhodozyma.
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