化学
细胞生物学
荧光显微镜
细胞毒性
荧光
生物
细胞
分子生物学
活体细胞成像
绿色荧光蛋白
α-突触核蛋白
细胞培养
生物物理学
染色
出处
期刊:Methods of Molecular Biology
日期:2021-01-01
卷期号:2322: 163-174
标识
DOI:10.1007/978-1-0716-1495-2_16
摘要
The budding yeast Saccharomyces cerevisiae is a model organism amenable both to genetic analysis and cell biology. Due to these advantages, yeast has provided platforms to examine the properties of pathogenic proteins involved in human diseases. The methods used to examine the cytotoxicity and intracellular localization of α-Synuclein, a human neuronal protein implicated in Parkinson's disease, using yeast have been described herein. These methods are readily accessible to researchers or graduate students unfamiliar with experiments using yeast and applicable to larger scale analyses, such as high-throughput genetic and chemical screenings.
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