类有机物
生物
旁分泌信号
内胚层
细胞生物学
细胞命运测定
解剖
细胞分化
受体
遗传学
转录因子
基因
作者
Ana C. Silva,Oriane B. Matthys,David A. Joy,M. Ariel Kauss,Vaishaali Natarajan,Michael H. Lai,Diwakar Turaga,Andrew P. Blair,Michael Alexanian,Benoit G. Bruneau,Todd C. McDevitt
出处
期刊:Cell Stem Cell
[Elsevier]
日期:2021-12-01
卷期号:28 (12): 2137-2152.e6
被引量:97
标识
DOI:10.1016/j.stem.2021.11.007
摘要
During embryogenesis, paracrine signaling between tissues in close proximity contributes to the determination of their respective cell fate(s) and development into functional organs. Organoids are in vitro models that mimic organ formation and cellular heterogeneity, but lack the paracrine input of surrounding tissues. Here, we describe a human multilineage iPSC-derived organoid that recapitulates cooperative cardiac and gut development and maturation, with extensive cellular and structural complexity in both tissues. We demonstrate that the presence of endoderm tissue (gut/intestine) in the organoids contributed to the development of cardiac tissue features characteristic of stages after heart tube formation, including cardiomyocyte expansion, compartmentalization, enrichment of atrial/nodal cells, myocardial compaction, and fetal-like functional maturation. Overall, this study demonstrates the ability to generate and mature cooperative tissues originating from different germ lineages within a single organoid model, an advance that will further the examination of multi-tissue interactions during development, physiological maturation, and disease.
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