脚手架
化学
纳米技术
电化学
三维细胞培养
石墨烯
电极
电化学电池
细胞培养
功能(生物学)
生物物理学
细胞
生物医学工程
细胞生物学
材料科学
生物化学
生物
医学
物理化学
遗传学
作者
Xuebo Hu,Yanling Liu,Wenjie Wang,Haiwei Zhang,Yu Qin,Shan Guo,Xinwei Zhang,Lei Fu,Wei‐Hua Huang
标识
DOI:10.1021/acs.analchem.7b03324
摘要
Current achievements on electrochemical monitoring of cells are often gained on two-dimensional (2D) substrates, which fail in mimicking the cellular environments and accurately reproducing the cellular functions within a three-dimensional (3D) tissue. In this regard, 3D scaffold concurrently integrated with the function of cell culture and electrochemical sensing is conceivably a promising platform to monitor cells in real time under their in vivo-like 3D microenvironments. However, it is particularly challenging to construct such a multifunctional scaffold platform. Herein, we developed a 3-aminophenylboronic acid (APBA) functionalized graphene foam (GF) network, which combines the biomimetic property of APBA with the mechanical and electrochemical properties of GF. Hence, the GF network can serve as a 3D scaffold to culture cells for a long period with high viability and simultaneously as an electrode for highly sensitive electrochemical sensing. This allows monitoring of gaseous messengers H2S released from the cells cultured on the 3D scaffold in real time. This work represents considerable progress in fabricating 3D cell culture scaffold with electrochemical properties, thereby facilitating future studies of physiologically relevant processes.
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