Enhanced fungal resistance in transgenic cotton expressing an endochitinase gene from Trichoderma virens

生物 转基因 交替链格孢 转基因作物 黑斑病 微生物学 植物 茄丝核菌 丝核菌 木霉菌 植物抗病性 链格孢 基因 遗传学
作者
Chandrakanth Emani,Juan M. García,Emily Lopata‐Finch,Marı́a J. Pozo,Pedro Uribe,Dong‐Jin Kim,Ganesan Sunilkumar,Douglas R. Cook,Charles M. Kenerley,Keerti S. Rathore
出处
期刊:Plant Biotechnology Journal [Wiley]
卷期号:1 (5): 321-336 被引量:152
标识
DOI:10.1046/j.1467-7652.2003.00029.x
摘要

Mycoparasitic fungi are proving to be rich sources of antifungal genes that can be utilized to genetically engineer important crops for resistance against fungal pathogens. We have transformed cotton and tobacco plants with a cDNA clone encoding a 42 kDa endochitinase from the mycoparasitic fungus, Trichoderma virens. Plants from 82 independently transformed callus lines of cotton were regenerated and analysed for transgene expression. Several primary transformants were identified with endochitinase activities that were significantly higher than the control values. Transgene integration and expression was confirmed by Southern and Northern blot analyses, respectively. The transgenic endochitinase activities were examined in the leaves of transgenic tobacco as well as in the leaves, roots, hypocotyls and seeds of transgenic cotton. Transgenic plants with elevated endochitinase activities also showed the expected 42 kDa endochitinase band in fluorescence, gel-based assays performed with the leaf extracts in both species. Homozygous T2 plants of the high endochitinase-expressing cotton lines were tested for disease resistance against a soil-borne pathogen, Rhizoctonia solani and a foliar pathogen, Alternaria alternata. Transgenic cotton plants showed significant resistance to both pathogens.

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