Study on methods of deproteinization from Phellinus baumii polysaccharide

Objective To study and compare different methods and influencing factors of deproteinization from Phellinus baumii polysaccharide. Methods Sevag method and TCA method were used to remove protein from crude polysaccharide of Phellinus baumii, respectively. Results The effect of TCA method on removing protein was superior to that of Sevag method,and the optimum conditions of removing protein with TCA were as follows: 30 min treating time, 5% trichloroacetic acid,3 times treatemt. Under these conditions the rate of protein-removed was 82% and the rate of polysaccharide-lost was 10.8%. Conclusion TCA method is the optimal mean for deproteinization from Phellinus baumii polysaccharide.